Molecular insights into the circadian clock in marine diatoms

Shufeng Zhang Yue Wu Lin Lin Dazhi Wang

Shufeng Zhang, Yue Wu, Lin Lin, Dazhi Wang. Molecular insights into the circadian clock in marine diatoms[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-021-1962-4
Citation: Shufeng Zhang, Yue Wu, Lin Lin, Dazhi Wang. Molecular insights into the circadian clock in marine diatoms[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-021-1962-4

doi: 10.1007/s13131-021-1962-4

Molecular insights into the circadian clock in marine diatoms

Funds: The National Natural Science Foundation of China under contract Nos. 41425021 and 41706131; the National Key Research and Development Program of China under contract No. 2017YFC1404302; Dazhi Wang was supported by the “Ten Thousand Talents Program” for leading talents in science and technological innovation.
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    †These authors contributed equally to this work.
  • Figure  1.  Growth curves of Skeletonema costatum and Phaeodactylum tricornutum under control (a and e), continuous light and dark (b and f), N-deplete and N-resupplied (c and g), P-deplete and P-resupplied (d and h) conditions. The red and blue rectangles indicate the sampling time for molecular analysis while the blue rectangles in c, d, g and h indicate the sampling time in the N- or P-resupplied groups. The red arrow indicates the time point of N or P resupply.

    Figure  2.  Conservative motif analysis of the core clock genes (CCA1, LHY, and TOC1) in S. costatum and P. tricornutum based on the databases SMART and Pfam. At: Arabidopsis, Pt: P. tricornutum, Sc: S. costatum.

    Figure  3.  Expression of CCA1, LHY, and TOC1 in Skeletonema costatum and Phaeodactylum tricornutum within 24 h. Expression of CCA1 in S. costatum and P. tricornutum under control (a-1 and b-1), continuous light (a-2 and b-2), continuous dark (a-3 and b-3), N-deplete (a-4 and b-4), N-resupplied (a-5 and b-5), P-deplete (a-6 and b-6) and P-resupplied (a-7 and b-7) conditions; expression of LHY in S. costatum and P. tricornutum under control (c-1 and d-1), continuous light (c-2 and d-2), continuous dark (c-3 and d-3), N-deplete (c-4 and d-4), N-resupplied (c-5 and d-5), P-deplete (c-6 and d-6) and P-resupplied (c-7 and d-7) conditions; and expression of TOC1 in S. costatum and P. tricornutum under control (e-1 and f-1), continuous light (e-2 and f-2), continuous dark (e-3 and f-3), N-deplete (e-4 and f-4), N-resupplied (e-5 and f-5), P-deplete (e-6 and f-6) and P-resupplied (e-7 and f-7) conditions.

    Figure  4.  The phylogenetic tree of CCA1, LHY, and TOC1. The phylogenetic tree of CCA1 (a), LHY (b), and TOC1 (c).

    Figure  5.  The speculative molecular model of the circadian clock in marine diatoms. Genes in the figure are shown in italics, proteins are shown in normal font. Words in red represent the genes that are identified in Skeletonema costatum and Phaeodactylum tricornutum; words in gray represent the genes that are not identified in S. costatum and P. tricornutum; words in green represent the genes that are identified in S. costatum; words in blue represent the genes that are identified in P. tricornutum. The core feedback loop of the circadian oscillator is highlighted in green lines; the white area represents daytime, while the shaded area represents nighttime. The environmental signals of the input pathway and the metabolic process of the output pathways showed in a previous study (Zhang et al., 2016).

    Table  1.   Circadian clock genes identified in the diatoms Skeletonema costatum, Phaeodactylum tricornutum, and other speciesa

    LocationGene nameEukaryotic algaeFlowering plantsb
    Skeletonema costatumPhaeodactylum tricornutumOstreococcus tauriChlamydomonas reinhardtiiSymbiodinium spp.Cyanidioschyzon merolaeArabidopsis thalianaOryza sativa
    Input pathwayPHYA+ndndnd+cnd++
    Core oscillatorCCA1+++ndndnd++
    Output pathwayCAB2++ndndndnd++
    Valve effectorLUX++ndndndnd+nd
    Note: a, Prokaryotes are not included in the table because they have completely different clock genes; b, only representative flowering plants are listed; c, unknown type. + indicates detected, nd indicates not detected. The content of this table refer to Martínez-García et al. (2000), Noordally and Millar (2015) and Zhang et al. (2016).
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