Turn off MathJax
Article Contents
Feng Liu, Xiaokang Lv, Tianqi Chu, Mengjie Wang, Wei Zhan, Bao Lou. Characterization of ghrelin mRNA expression in fasting Larimichthys crocea juveniles[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1692-z
Citation: Feng Liu, Xiaokang Lv, Tianqi Chu, Mengjie Wang, Wei Zhan, Bao Lou. Characterization of ghrelin mRNA expression in fasting Larimichthys crocea juveniles[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1692-z

Characterization of ghrelin mRNA expression in fasting Larimichthys crocea juveniles

doi: 10.1007/s13131-020-1692-z
Funds:  The Key Research and Development Project of Zhejiang Province under contract Nos. 2016C02055-7 and 2017C02013.
More Information
  • Corresponding author: E-mail: loubao6577@163.com
  • Received Date: 2019-09-30
  • Accepted Date: 2019-11-01
  • Available Online: 2021-04-01
  • Larimichthys crocea is a marine fish species cultured in China. Short-term starvation is often applied to improve the quality of cultured L. crocea, and the expression of ghrelin in tissues of stomach, muscle, brain, intestines, liver, and kidney, involved in starvation response, under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L. crocea juveniles. The ghrelin expression was tissue-specific, and expression was significantly higher in the stomach compared to other tissues (P<0.01). Additionally, ghrelin expression in different tissues changed along with prolongation of fasting. In the stomach, ghrelin expression levels increased gradually at the beginning of the fast, and then declined after eight days of fasting. Gene expression in the brain and intestines increased at the beginning of the fast, and then decreased with longer fasting time. Interestingly, ghrelin expression declined at the beginning of the fast, then increased with longer fasting in the kidneys and muscles. These results suggest that ghrelin is involved in starvation response in L. crocea juveniles. This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L. crocea juveniles.
  • loading
  • [1]
    Amole N, Unniappan S. 2009. Fasting induces preproghrelin mRNA expression in the brain and gut of zebrafish, Danio rerio. General and Comparative Endocrinology, 161(1): 133–137. doi: 10.1016/j.ygcen.2008.11.002
    Arvat E, Di Vito L, Broglio F, et al. 2000. Preliminary evidence that Ghrelin, the natural GH secretagogue (GHS)-receptor ligand, strongly stimulates GH secretion in humans. Journal of Endocrinological Investigation, 23(8): 493–495. doi: 10.1007/bf03343763
    Cui Wentao. 2013. Cloning and distribution of three kinds of gastrointestinal peptide cDNAs in large yellow croaker (Pseudosciaena crocea) (in Chinese) [dissertation]. Zhoushan: Zhejiang Ocean University
    Diéguez C, da Boit K, Novelle M G, et al. 2010. New insights in ghrelin orexigenic effect. Frontiers of Hormone Research, 38: 196–205. doi: 10.1159/000318511
    Feng Ke, Zhang Guirong, Wei Kaijian, et al. 2013. Molecular cloning, tissue distribution, and ontogenetic expression of ghrelin and regulation of expression by fasting and refeeding in the grass carp (Ctenopharyngodon idellus). Journal of Experimental Zoology Part A: Ecological & Integrative Physiology, 319(4): 202–212. doi: 10.1002/jez.1784
    Feng Zhaoxin. 1979. Ichthyology (in Chinese). Beijing: Agricultural Press House, 217
    Fox B K, Breves J P, Hirano T, et al. 2009. Effects of short- and long-term fasting on plasma and stomach ghrelin, and the growth hormone/insulin-like growth factor I axis in the tilapia, Oreochromis mossambicus. Domestic Animal Endocrinology, 37(1): 1–11. doi: 10.1016/j.domaniend.2009.01.001
    Gao Fengying, Wang Huan, Lu Maixin, et al. 2010. cDNA cloning and expression characterization of ghrelin of Zanzibar tilapia, Oreochromis hornorum. Journal of Huazhong Agricultural University (in Chinese), 29(6): 752–757
    Ginés R, Palicio M, Zamorano M J, et al. 2002. Starvation before slaughtering as a tool to keep freshness attributes in gilthead sea bream (Sparus aurata). Aquaculture International, 10(5): 379–389. doi: 10.1023/A:1023365025292
    Heijboer A C, van den Hoek A M, Parlevliet E T, et al. 2006. Ghrelin differentially affects hepatic and peripheral insulin sensitivity in mice. Diabetologia, 49(4): 732–738. doi: 10.1007/s00125–006-0138–2
    Hosoda H, Kojima M, Matsuo H, et al. 2000. Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue. Biochemical and Biophysical Research Communications, 279(3): 909–913. doi: 10.1006/bbrc.2000.4039
    Jönsson E. 2013. The role of ghrelin in energy balance regulation in fish. General and Comparative Endocrinology, 187: 79–85. doi: 10.1016/j.ygcen.2013.03.013
    Kaiya H, Kojima M, Hosoda H, et al. 2003a. Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout. Endocrinology, 144(12): 5215–5226. doi: 10.1210/en.2003–1085
    Kaiya H, Kojima M, Hosoda H, et al. 2003b. Amidated fish ghrelin: purification, cDNA cloning in the Japanese eel and its biological activity. Journal of Endocrinology, 176(3): 415–423. doi: 10.1677/joe.0.1760415
    Kaiya H, Kojima M, Hosoda H, et al. 2003c. Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 135(3): 421–429. doi: 10.1016/s1096–4959(03)00109-x
    Kaiya H, Small B C, Bilodeau A L, et al. 2005. Purification, cDNA cloning, and characterization of ghrelin in channel catfish, Ictalurus punctatus. General and Comparative Endocrinology, 143(3): 201–210. doi: 10.1016/j.ygcen.2005.03.012
    Karapanagiotou E M, Polyzos A, Dilana K D, et al. 2009. Increased serum levels of ghrelin at diagnosis mediate body weight loss in non-small cell lung cancer (NSCLC) patients. Lung Cancer, 66(3): 393–398. doi: 10.1016/j.lungcan.2009.02.006
    Kojima M, Hosoda H, Date Y, et al. 1999. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature, 402(6762): 656–660. doi: 10.1038/45230
    Kono T, Kitao Y, Sonoda K, et al. 2008. Identification and expression analysis of ghrelin gene in common carp Cyprinus carpio. Fisheries Science, 74(3): 603–612. doi: 10.1111/j.1444–2906.2008.01564.x
    Li Wenjie, Ai Qinghui, Mai Kangsen, et al. 2013. Effects of dietary amino acid patterns on growth and protein metabolism of large yellow croaker (Larimichthys crocea) larvae. Aquaculture, 406–407: 1–8. doi: 10.1016/j.aquaculture.2013.04.029
    Ma Xilan, Zhang Yong, Liu Yun, et al. 2009. Effect of different fasting stages on ghrelin expression in Black Sea Bream, Acanthopagrus schlegeli. Oceanologia et Limnologia Sinica (in Chinese), 40(3): 313–318
    Murashita K, Kurokawa T, Nilsen T O, et al. 2009. Ghrelin, cholecystokinin, and peptide YY in Atlantic salmon (Salmo salar): molecular cloning and tissue expression. General and Comparative Endocrinology, 160(3): 223–235. doi: 10.1016/j.ygcen.2008.11.024
    Olsson C, Holbrook J D, Bompadre G, et al. 2008. Identification of genes for the ghrelin and motilin receptors and a novel related gene in fish, and stimulation of intestinal motility in zebrafish (Danio rerio) by ghrelin and motilin. General and Comparative Endocrinology, 155(1): 217–226. doi: 10.1016/j.ygcen.2007.05.016
    Parhar I S, Sato H, Sakuma Y. 2003. Ghrelin gene in cichlid fish is modulated by sex and development. Biochemical and Biophysical Research Communications, 305(1): 169–175. doi: 10.1016/s0006–291x(03)00729–0
    Peddu S C, Breves J P, Kaiya H, et al. 2009. Pre- and postprandial effects on ghrelin signaling in the brain and on the GH/IGF-I axis in the Mozambique tilapia (Oreochromis mossambicus). General and Comparative Endocrinology, 161(3): 412–418. doi: 10.1016/j.ygcen.2009.02.008
    Pusztai P, Sarman B, Ruzicska E, et al. 2008. Ghrelin: a new peptide regulating the neurohormonal system, energy homeostasis and glucose metabolism. Diabetes/Metabolism Research and Reviews, 24(5): 343–352. doi: 10.1002/dmrr.830
    Riley L G, Fox B K, Breves J P, et al. 2008. Absence of effects of short-term fasting on plasma ghrelin and brain expression of ghrelin receptors in the Tilapia, Oreochromis mossambicus. Zoological Science, 25(8): 821–827. doi: 10.2108/zsj.25.821
    Terova G, Rimoldi S, Bernardini G, et al. 2008. Sea bass ghrelin: molecular cloning and mRNA quantification during fasting and refeeding. General and Comparative Endocrinology, 155(2): 341–351. doi: 10.1016/j.ygcen.2007.05.028
    Thakur D P, Morioka K, Itoh Y, et al. 2003. Lipid composition and deposition of cultured yellowtail Seriola quinqueradiata muscle at different anatomical locations in relation to meat texture. Fisheries Science, 69(3): 487–494. doi: 10.1046/j.1444–2906.2003.00649.x
    Unniappan S, Canosa L F, Peter R E. 2004. Orexigenic actions of ghrelin in goldfish: feeding-induced changes in brain and gut mRNA expression and serum levels, and responses to central and peripheral injections. Neuroendocrinology, 79(2): 100–108. doi: 10.1159/000076634
    Volkoff H. 2015. Cloning, tissue distribution and effects of fasting on mRNA expression levels of leptin and ghrelin in red-bellied piranha (Pygocentrus nattereri). General and Comparative Endocrinology, 217–218: 20–27. doi: 10.1016/j.ygcen.2015.05.004
    Volkoff H, Peter R E. 2006. Feeding behavior of fish and its control. Zebrafish, 3(2): 131–140. doi: 10.1089/zeb.2006.3.131
    Wang Le, Shi Xiaofeng, Su Yongquan, et al. 2012. Loss of genetic diversity in the cultured stocks of the large yellow croaker, Larimichthys crocea, revealed by microsatellites. International Journal of Molecular Sciences, 13(5): 5584–5597. doi: 10.3390/ijms13055584
    Wang Qianfeng, Shen Weiliang, Liu Cheng, et al. 2017. Effects of multi-environmental factors on physiological and biochemical responses of large yellow croaker, Larimichthys crocea. Chemosphere, 184: 907–915. doi: 10.1016/j.chemosphere.2017.06.043
    Wren A M, Bloom S R. 2007. Gut hormones and appetite control. Gastroenterology, 132(6): 2116–2130. doi: 10.1053/j.gastro.2007.03.048
    Wren A M, Small C J, Ward H L, et al. 2000. The novel hypothalamic peptide Ghrelin stimulates food intake and growth hormone secretion. Endocrinology, 141(11): 4325–4328. doi: 10.1210/endo.141.11.7873
    Xing Shujuan, Sun Ruijian, Pan Xiaoyi, et al. 2016. Effects of dietary carbohydrate-to-lipid ratio on growth performance, body composition, digestive enzyme activities, and hepatic enzyme activities in juvenile large yellow croaker, Larimichthys crocea. Journal of the World Aquaculture Society, 47(2): 297–307. doi: 10.1111/jwas.12259
    Xu Meiyu, Volkoff H. 2009. Molecular characterization of ghrelin and gastrin-releasing peptide in Atlantic cod (Gadus morhua): Cloning, localization, developmental profile and role in food intake regulation. General and Comparative Endocrinology, 160(3): 250–258. doi: 10.1016/j.ygcen.2008.12.004
    Ye Hua, Liu Yang, Liu Xiande, et al. 2014. Genetic mapping and QTL analysis of growth traits in the large yellow croaker Larimichthys crocea. Marine Biotechnology, 16(6): 729–738. doi: 10.1007/s10126–014-9590-z
    Yeung C M, Chan C B, Woo N Y, et al. 2006. Seabream ghrelin: cDNA cloning, genomic organization and promoter studies. Journal of Endocrinology, 189(2): 365–379. doi: 10.1677/joe.1.06593
    Zhang Hanle, Sun Ruijian, Xu Wei, et al. 2016a. Dietary manganese requirement of juvenile large yellow croaker Larimichthys crocea (Richardson, 1846). Aquaculture, 450: 74–79. doi: 10.1016/j.aquaculture.2015.07.010
    Zhang Zhenyu, Wang Qiurong, Ye Kun, et al. 2016b. Effect of fasting on body composition, muscle fatty acid profiles and serum biochemical parameters of large yellow croaker (Larimichthys crocea). Journal of Fisheries of China (in Chinese), 40(9): 1440–1450
    Zhou Chaowei, Zhang Xingdong, Liu Tao, et al. 2014. Schizothorax davidi ghrelin: cDNA cloning, tissue distribution and indication for its stimulatory character in food intake. Gene, 534(1): 72–77. doi: 10.1016/j.gene.2013.10
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(1)

    Article Metrics

    Article views (159) PDF downloads(7) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint